Recommend the nonstructural protein and paired antibody of Zika virus
1: Non structural protein 1 (NS1) has high diagnostic specificity. Through the strategy of gene sequence optimization, site directed mutation and so on, the recombinant antigen was developed in cooperation with many domestic and foreign scientific research institutions, and has been fully verified in clinical practice. A large number of experimental data confirmed that the recombinant antigen only combined with Zika positive serum, without cross with dengue, yellow fever, West Nile, JE, chikungunya, falciparum malaria and other positive serum, which can achieve the purpose of differential diagnosis, which is the biggest advantage and selling point of the recombinant antigen. The basic model of double antigen sandwich detection antibody can be designed, which can be applied to enzyme labeled diagnostic reagents, colloidal gold diagnostic reagents, fluorescence diagnostic reagents and other methodological diagnostic reagents.
2: Monoclonal antibody (paired antibody) to nonstructural protein 1 (NS1) of Zika virus
The McAb has a strong diagnostic specificity and sensitivity, and has no cross with dengue, yellow fever, West Nile, JE, chikungunya, falciparum malaria and other positive sera. The detection limit of double antibody sandwich ELISA based on the paired antibody can reach 50pg / ml, the detection limit of colloidal gold method can reach 5ng / ml, and the detection limit of fluorescence method can reach 50pg / ml.
Three: one stop service provided by Jieyi biological
We promise to provide you with free samples for trial use while high-quality raw materials, and provide guidance throughout the process. No matter you are in the use of enzyme, colloidal gold or fluorescence method, you can not achieve the expected goal. We provide free assistance services, so that you can worry about it and put the products into the market as soon as possible.
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5: Application mode diagram of enzyme labeling and effect diagram of colloidal gold test
A. purification of recombinant NS1 protein; B. identification of Western blot
Double antigen sandwich ELISA pattern
Zika IgM ELISA pattern (mac-elisa)
Zika IgG ELISA pattern (indirect ELISA)